Biotin Anti-Mouse IgG2a Rabbit Monoclonal Antibody [Clone RM219], 31-1098-02
![Biotin Anti-Mouse IgG2a Rabbit Monoclonal Antibody [Clone RM219], 31-1098-02](/upload/image/products/Fig3-WB-IgG2a_E.png)
![Biotin Anti-Mouse IgG2a Rabbit Monoclonal Antibody [Clone RM219], 31-1098-02](/upload/image/products/Fig2-MsIgG2a_E.png)
![Biotin Anti-Mouse IgG2a Rabbit Monoclonal Antibody [Clone RM219], 31-1098-02](/upload/image/products/Fig1-MsIgG2a_E.png)
Key features and details
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Product Details
| Product Name | Biotin Anti-Mouse IgG2a Rabbit Monoclonal Antibody, Clone RM219 |
| Product Description | Biotinylated Rabbit monoclonal to Mouse IgG2a |
| Catalog No. | 31-1098-02 |
| Clone Name | RM219 |
| Product Data Sheet | Biotin Anti-Mouse IgG2a Rabbit Monoclonal Antibody Data Sheet RM219 |
| Specificity | RM219 reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. |
| Immunogen | Mouse IgG2a |
| Application | ELISA Western Blot (WB) (nonreduced only) |
| Species Reactivity | Mouse |
| Conjugate | Biotin |
| Intended Use | Research Use Only |
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| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 50 μg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ELISA: 0.005 μg/mL – 0.2 μg/mL; Western Blot (WB): 0.5 μg/mL - 2 μg/mL. |
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 | ELISA of mouse immunoglobulins shows RM219 reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM219 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
 | A titer ELISA of mouse IgG2a. The plate was coated with different amounts of mouse IgG2a. A serial dilution of RM219 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
 | Western blot of nonreduced (-) and reduced (+) mouse IgG2a, using 0.5 μg/mL of RevMAb Clone RM219. This antibody reacts to nonreduced IgG2a (~150 kDa). |
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