Biotin Anti-Mouse IgG3 Rabbit Monoclonal Antibody [Clone RM218], 31-1094-02
![Biotin Anti-Mouse IgG3 Rabbit Monoclonal Antibody [Clone RM218], 31-1094-02](/upload/image/products/RM218_ELISA_multiple_Igs.png)
![Biotin Anti-Mouse IgG3 Rabbit Monoclonal Antibody [Clone RM218], 31-1094-02](/upload/image/products/RM218_ELISA_MsIgG3.png)
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CAT.NO. : 31-1094-02
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Product Details
Product Details
| Product Name | Biotin Anti-Mouse IgG3 Rabbit Monoclonal Antibody, Clone RM218 |
| Product Description | Biotinylated Rabbit monoclonal to Mouse IgG3 |
| Catalog No. | 31-1094-02 |
| Clone Name | RM218 |
| Product Data Sheet | Biotin Anti-Mouse IgG3 Rabbit Monoclonal Antibody Data Sheet RM218 |
| Specificity | RM218 reacts to the Fab region of mouse IgG3. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG. |
| Immunogen | Mouse IgG3 |
| Application | ELISA Western Blot (WB) |
| Species Reactivity | Mouse |
| Conjugate | Biotin |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 50 μg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ELISA: 0.005 μg/mL - 0.2 μg/mL; Western Blot (WB):0.1 μg/mL - 0.5 μg/mL. |
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Product Images
 | ELISA of mouse immunoglobulins shows RM218 reacts to both mouse IgG3κ and IgG3λ; No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins.200 ng/mL, 50 ng/mL, or 10 ng/mL of RM218 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
 | A titer ELISA of mouse IgG3. The plate was coated with different amounts of mouse IgG3. A serial dilution of RM218 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit Ig Gas the secondary antibody. |
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