Biotin Anti-Mouse Immunoglobulin Lambda Light Chain Rabbit Monoclonal Antibody [Clone RM110], 31-1006-02
Product Name | Biotin Anti-Mouse Immunoglobulin Lambda Light Chain Rabbit Monoclonal Antibody, Clone RM110 |
Product Description | Biotinylated Rabbit monoclonal to Mouse Ig Lambda Light Chain |
Catalog No. | 31-1006-02 |
Clone Name | RM110 |
Product Data Sheet | Biotin Anti-Mouse Ig Lambda Rabbit Monoclonal Antibody Data Sheet RM110 |
Specificity | RM110 reacts to the lambda light chain of mouse immunoglobulins. No cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG. The Fc region of RM110 has been engineered to eliminate Fc receptor binding. |
Immunogen | Mouse IgM lambda |
Application | ELISA Western Blot (WB) (nonreduced) |
Species Reactivity | Mouse |
Conjugate | Biotin |
Intended Use | Research Use Only |
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Form | Liquid |
Storage Instructions | Store at -20.0°C |
Stability | Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Volume/Item Size | 50 µg |
Concentration | 1.0 mg/mL |
Purity | Protein A affinity purified from an animal origin–free culture supernatant |
Clone Type | Monoclonal |
Isotype | Rabbit IgG |
Usage | ELISA: 0.005 µg/mL - 0.2 µg/mL Western Blot (WB): 0.1 µg/mL - 0.5 µg/mL |
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![]() | Western blot of nonreduced (-) and reduced (+) mouse IgG1κ and IgG1λ (20 ng/lane), using 0.2 µg/mL of RevMAb Clone RM110. This antibody reacts to nonreduced IgG1λ. |
![]() | ELISA of mouse immunoglobulins shows RM110 reacts to the lambda light chain of mouse immunoglobulins. No cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM110 was used as the primary antibody. Analkaline phosphatase conjugated anti-rabbit |
![]() | A titer ELISA of mouse IgG1λ. The plate was coated with different amounts of mouse IgG1λ. A serial dilution of RM110 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
