C9RANT Mouse Monoclonal Antibody(ARA714)
Key features and details
- Reactivity:Human, Mouse, Rat
- Application:WB, IHC, ICC/IF
- Host:Mouse
- Clonality:Monoclonal
- lsotype: IgG
- Target:C9RANT
-
Brand:
CAT.NO. : ARA6482
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Product Details
Background
C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). FTLD-TDP has five subtypes: Sporadic FTLD, GRN mutation FTLD, TARDBP mutation FTLD, VCP mutation FTLD and C9ORF72 mutation FTLD. The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa.
Application
|
Application |
Dilution Ratio |
|
WB |
1:500 - 1:1000 |
|
IHC |
1:500 - 1:2000 |
|
IF/ICC |
1:200 - 1:800 |
Overview
|
Species Reactivity |
Human, Mouse, Rat |
|
Immunogen |
Recombinant human C9orf72 protein |
|
Host / Isotype |
Mouse / IgG2a |
|
Antibody Class |
Monoclonal |
|
Alternative Names |
Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT |
|
Calculated Molecular Weight |
481 aa, 54 kDa |
|
Observed Molecular Weight |
55 kDa |
|
GenBank Protein ID |
BC020851 |
|
Gene Name |
C9orf72 |
|
Gene ID (NCBI) |
203228 |
|
Conjugation |
Unconjugated |
|
Form |
Liquid |
|
Purification Method |
Protein A purification |
|
UNIPROT ID |
Q96LT7 |
|
Storage Buffer |
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Data
Neuro-2a cells were subjected to SDS PAGE followed by western blot with C9RANT Mouse Monoclonal Antibody(ARA714) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human brain using C9RANT Mouse Monoclonal Antibody(ARA714) at dilution of 1:50 (under 10x lens).
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using C9RANT Mouse Monoclonal Antibody(ARA714) at dilution of 1:400 and Flour 488-Conjugated Goat Anti-Mouse IgG(H+L) .
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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