Caspase-3 Rabbit Monoclonal Antibody(ARB583)
Key features and details
- Reactivity:Human
- Application:IHC
- Host:Rabbit
- Clonality:Monoclonal
- Target:Caspase-3
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Brand:
CAT.NO. : ARB6875
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Size:
Trail, Bulk size or Custom requests Please contact us
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Product Details
Background
Caspase-3 is a key executioner cysteine-aspartic protease in the caspase family, essential for mediating apoptosis. It exists as an inactive zymogen (pro-caspase-3) and is activated by proteolytic cleavage via initiator caspases (caspase-8, -9) in response to extrinsic (death receptor) or intrinsic (mitochondrial) apoptotic signals. Caspase-3 is mainly expressed in tissues with high cellular turnover or apoptotic activity, including immune organs (thymus, spleen, bone marrow), developing tissues (embryonic structures, placenta), epithelia (intestinal mucosa, skin) and glands (mammary, prostate). Caspase-3, particularly its cleaved active form, serves as a gold-standard biomarker for detecting apoptosis in clinical histopathology. Immunohistochemical (IHC) staining for cleaved Caspase-3 is routinely used to confirm apoptotic activity in tumors post-therapy (chemotherapy/radiotherapy), where activation correlates with treatment efficacy.
Application
|
Application |
Dilution Ratio |
|
IHC-P |
1:100-1:200 |
Overview
|
Predicted Molecular Wt |
32kDa |
|
Species Cross-reactivity |
Human |
|
Applications |
IHC-P |
|
Purity |
ProA affinity purified IgG |
|
Form |
Liquid |
|
Swissprot ID |
P42574 |
|
Subcellular location |
Cytoplasm and nucleus |
|
Recommended Method |
Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18℃-25℃) for 30 minutes. |
|
Immunogen |
Synthetic peptide. |
|
Storage Buffer |
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
Data
Immunohistochemical staining of human tonsil tissue using Caspase-3 Rabbit Monoclonal Antibody(ARB583)
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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