CD15 Mouse Monoclonal Antibody(ARB853)
Key features and details
- Target:
- Clone ID:
- Host:
- Molecular Weight:
- Purity:
- Species Cross-reactivity:
- Applications:
- Swissprot ID:
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Brand:
CAT.NO. : ARB6643
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Product Details
Background
CD15 is present on greater than 90% of granulocytes including neutrophils and eosinophils, and to a lesser degree, on monocytes. CD15 is expressed in Reed-Sternberg cells of Hodgkin's disease (of the nodular sclerosis, mixed cellularity and lymphocytedepleted subtypes), and certain types of epithelial cells. It is generally agreed that the Reed-Sternberg cell variants in lymphocytepredominant Hodgkin's disease are not reactive with CD15. Positive staining for CD15 combined with a negative reaction for lymphocytic markers may provide support for Hodgkin’s disease.
Overview
| Target | CD15 |
| Host Species | mouse monoclonal antibody |
| Molecular Weight | 59 kDa |
| Purity | Affinity purified IgM |
| Species Cross-reactivity | Human |
| Form | Liquid |
| Applications | IHC-P |
| Swissprot ID | P22083 |
| Immunogen | Immunized with U-937 histiocytic cell line |
| Storage Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
| Storage Conditions | -25°C to -18°C |
| Dilutions | IHC-P: 1:100-1:200 |
| Subcellular Location | Membrane |
| Recommended Method | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT for 30 minutes |
Data
Immunohistochemical staining of human Hodgkin lymphoma tissue sections using CD15 Mouse Monoclonal Antibody (ARB853).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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