CD184 Rabbit Polyclonal Antibody

Key features and details

Reactivity:Human, Mouse, Rat
Application:WB,IHC
Host:Rabbit
Clonality:Polyclonal
Target:CD184

Brand:

CAT.NO. : ARA6637

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Size:

100μL

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Product Details

Background

CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.

Application

Application	Dilution Ratio
WB	1:1000 - 1:2000
IHC	1:100 - 1:200

Overview

Product Description	Rabbit polyclonal antibody to CD184
Immunogen	KLH - conjugated synthetic peptide encompassing a sequence of human CD184. The exact sequence is proprietary.
Purification Method	The antibody was purified by immunogen affinity chromatography
Clonality	Polyclonal
Product Form	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide
Gene Name	CXCR4
Related Names	C - X - C chemokine receptor type 4; CXC - R4; CXCR - 4; FB22; Fusin; HM89; LCR1; Leukocyte - derived seven transmembrane domain receptor; LESTR; Lipopolysaccharide - associated protein 3; LAP - 3; LPS - associated protein 3; NPYRL; Stromal cell - derived factor 1 receptor; SDF - 1 receptor; CD184
Gene ID (Human)	7852
Protein ID (Human)	P61073

Data

Western blot analysis of CD184 expression in Hela (A), rat brain (B) whole cell lysates. (Predicted band size: 39 kD; Observed band size: 50 kD)

Immunohistochemical analysis of CD184 staining in human breast cancer,human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Research Use Only

For Research Use Only. Not for use in diagnostic procedures.

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