CD22 Rabbit Monoclonal Antibody(ARA824)
Key features and details
- Reactivity:Human
- Application:WB, IHC - P, IF
- Host:Rabbit
- Clonality:Monoclonal
- lsotype:IgG
- Target:CD22
-
Brand:
CAT.NO. : ARA6616
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Product Details
Background
CD22 antigen appears early in B-cell lymphocyte differentiation at approximately the same stage as the CD19 antigen. Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. CD22 may be a useful marker for phenotyping mature leukemias, as CD22 membrane expression has been shown to be limited to the late differentiation stages between mature B cells (CD22+) and plasma cells (CD22-). CD22 is also strongly expressed in hairy cell leukemia.
Application
|
Applications |
Tested Dilution |
|
WB |
1:1,000 |
|
IHC-P |
1:1,000 |
|
IF-Tissue |
1:200 |
Overview
|
Immunogen |
Synthetic peptide within Human CD22 aa 1 - 100 (N terminal). |
|
Species Reactivity |
Human |
|
Validated Applications |
WB, IHC - P, IF - Tissue |
|
Molecular Weight |
Predicted band size: 95 kDa |
|
Positive Controls |
Daudi cell lysates, human B - cell lymphoma tissue, human tonsil tissue, human spleen tissue. |
|
Conjugation |
unconjugated |
|
RRID |
AB_3072279 |
|
Form |
Liquid |
|
Concentration |
1μg/μl |
|
Storage Buffer |
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
|
Isotype |
IgG |
|
Purification Method |
Protein A affinity purified. |
Data
Western blot analysis of CD22 on Daudi cell lysates with Rabbit anti-CD22 antibody (ARA6616) at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-CD22 antibody (ARA6616) at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD22 antibody (ARA6616) at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD22 antibody (ARA6616) at 1/1,000 dilution.
Application: IF-Tissue
Species: Human
Site: Spleen
Sample: Paraffin-embedded section
Antibody concentration: 1/200
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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