CD74 Mouse Monoclonal Antibody(ARA903)

HLA class II histocompatibility antigen gamma chain also known as HLA-DR antigens-associated invariant chain or CD74 (Cluster of Differentiation 74), is a protein that in humans is encoded by the CD74 gene. The invariant chain (Abbreviated Ii) is a polypeptide which plays a critical role in antigen presentation. It is involved in the formation and transport of MHC class II peptide complexes for the generation of CD4+ T cell responses. The cell surface form of the invariant chain is known as CD74. CD74 is a cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF). The nascent MHC class II protein in the rough endoplasmic reticulum (RER) binds a segment of the invariant chain (Ii; a trimer) in order to shape the peptide-binding groove and prevent the formation of a closed conformation. The invariant chain also facilitates the export of MHC class II from the RER in a vesicle. The signal for endosomal targeting resides in the cytoplasmic tail of the invariant chain. This fuses with a late endosome containing the endocytosed antigen proteins (from the exogenous pathway). Binding to Ii ensures that no antigen peptides from the endogenous pathway meant for MHC class I molecules accidentally bind to the groove of MHC class II molecules. The Ii is then cleaved by cathepsin S (cathepsin L in cortical thymic epithelial cells), leaving only a small fragment called CLIP remaining bound to the groove of MHC class II molecules. The rest of the Ii is degraded. CLIP blocks peptide-binding until HLA-DM interacts with MHC II, releasing CLIP and allowing other peptides to bind. In some cases, CLIP dissociates without any further molecular interactions, but in other cases the binding to the MHC is more stable. The stable MHC class II + antigen complex is then presented on the cell surface. Without CLIP, MHC class II aggregates disassemble and/or denature in the endosomes, and proper antigen presentation is impaired.

Application	Dilution Ratio
IHC-P	1:200-1:1000

Antibody Type	Recombinant Mouse Monoclonal Antibody
Immunogen	Synthetic peptide
Species Reactivity	Human
Validated Applications	WB, IHC-P, IF-Cell, IF-Tissue
Molecular Weight	Predicted band size: 33 kDa
Positive Control	Daudi cell lysate, Raji cell lysate, LOVO, human B-cell lymphoma tissue, human tonsil tissue, human spleen tissue, human prostate cancer tissue
Conjugation	unconjugated
Form	Liquid
Concentration	2μg/μl
Storage Buffer	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide
Isotype	IgG1
Purification Method	Protein A affinity purified

Immunohistochemical staining of human B-cell lymphoma tissue using CD74 Mouse Monoclonal Antibody(ARA903)

Immunohistochemical staining of human spleen tissue using CD74 Mouse Monoclonal Antibody(ARA903)

Immunohistochemical staining of human prostate cancer tissue using CD74 Mouse Monoclonal Antibody(ARA903)

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

For Research Use Only. Not for use in diagnostic procedures.