Cell Cycle Marker Antibody Panel (Cyclin B1, Cyclin E)

Key features and details

  • 产品描述: The cell cycle is controlled by numerous mechanisms ensuring correct cell division. Cell division consists of two consecutive processes, mainly characterized by DNA replication (S phase), proceeding after G1 interphase; and segregation of replicated chromosomes into two separate cells (M phase), following G2 phase. Cyclin E is upregulated during G1/S progression whereas Cyclin A and Cyclin B are expressed highly during G2/M progression. Phosphorylated Histone H3 is a distinct marker for Mitosis and often used in determining cells undergoing M-phase in a proliferating populations. Arigo’s Cell Cycle Phase Marker Panel provides a useful tool as markers for cells undergoing different phases in cell cycle. Vermeulen et al. (2003) Cell Prolif 36:131-149 Rahman et al. (2010) Cell Cycle 9: 22-27 Hans and Dimitrov (2001) Oncogene 24: 3021-3027
  • 靶点名称: Cell Cycle Marker
  • 別名: Cell Cycle Marker antibody; Histone H3 phospho (Ser10) antibody; Cyclin E1 antibody; Cyclin B1 antibody
  • Brand:
CAT.NO. : ARG30139
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Product Details
内含物
货号内含物名称宿主克隆性反应应用包装
ARG52927anti-Cyclin E1 antibodyRabbit pAbHuIHC-P250 μl
ARG62456anti-Cyclin B1 antibody [V152]Mouse mAbHuFACS, ICC/IF, IHC-Fr, IHC-P, WB250 μl
ARG51679anti-Histone H3 phospho (Ser10) antibodyRabbit pAbHu, Ms, Rat, CydippidsFACS, ICC/IF, IHC-P, WB50 μl
ARG65350Goat anti-Mouse IgG antibody (HRP)Goat pAbMsELISA, IHC-P, WB50 μl
ARG65351Goat anti-Rabbit IgG antibody (HRP)Goat pAbRbELISA, IHC-P, WB50 μl
概述
产品描述The cell cycle is controlled by numerous mechanisms ensuring correct cell division. Cell division consists of two consecutive processes, mainly characterized by DNA replication (S phase), proceeding after G1 interphase; and segregation of replicated chromosomes into two separate cells (M phase), following G2 phase. Cyclin E is upregulated during G1/S progression whereas Cyclin A and Cyclin B are expressed highly during G2/M progression. Phosphorylated Histone H3 is a distinct marker for Mitosis and often used in determining cells undergoing M-phase in a proliferating populations.

Arigo’s Cell Cycle Phase Marker Panel provides a useful tool as markers for cells undergoing different phases in cell cycle.

Vermeulen et al. (2003) Cell Prolif 36:131-149
Rahman et al. (2010) Cell Cycle 9: 22-27
Hans and Dimitrov (2001) Oncogene 24: 3021-3027
靶点名称Cell Cycle Marker
別名Cell Cycle Marker antibody; Histone H3 phospho (Ser10) antibody; Cyclin E1 antibody; Cyclin B1 antibody
属性
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
全名Antibody Panel for Cell Cycle Marker (Cyclin B1, Cyclin E)
研究领域Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody
检测图片 (8)
  • ARG62456 anti-Cyclin B1 antibody [V152] WB image

    Western blot: 20 µl of HeLa cell lysate stained with ARG62456 anti-Cyclin B1 antibody [V152] at 1 µg/ml dilution.

  • ARG51679 anti-Histone H3 phospho (Ser10) antibody ICC/IF image

    Immunofluorescence: Hydractinia stained with ARG51679 anti-Histone H3 phospho (Ser10) antibody.

    From Gonzalo Quiroga-Artigas et al. Open Biol. (2022), doi: 10.1098/rsob.220120, Fig. 3.

  • ARG51679 anti-Histone H3 phospho (Ser10) antibody ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG51679 anti-Histone H3 phospho (Ser10) antibody at 1:100 dilution. Left: primary antibody (orange). Middle: DAPI (blue). Right: Merge.

    Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC).

  • ARG51679 anti-Histone H3.1 phospho (Ser10) antibody WB image

    Western Blot: extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells stained with anti-Histone H3.1 (phospho Ser10) antibody ARG51679.

  • ARG51679 anti-Histone H3.1 phospho (Ser10) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with anti-Histone H3.1 (phospho Ser10) antibody ARG51679.

  • ARG51679 anti-Histone H3.1 phospho (Ser10) antibody IHC-P image

    Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-Histone H3.1 (phospho Ser10) antibody ARG51679 (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51679 anti-Histone H3.1 phospho (Ser10) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with anti-Histone H3.1 (phospho Ser10) antibody ARG51679.

  • ARG52927 anti-Cyclin E antibody IHC-P image

    Immunohistochemistry: Human Breast Carcinoma stained with Cyclin E antibody (ARG52927)

参考文献

KDF1 Promoted Proliferation, Migration and Invasion of Lung Adenocarcinoma Cells through Activating STAT3 and AKT Pathway

ARG65350: WB / 

Yi-Qing Guo et al.
Biomedicines.,  (2023)

publication_link

 

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GNL3 is an evolutionarily conserved stem cell gene influencing cell proliferation, animal growth and regeneration in the hydrozoan Hydractinia

ARG51679: ICC-IF / Hydractinia

Gonzalo Quiroga-Artigas et al.
Open Biol.,  (2022)

publication_link

 

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Silencing of AURKA augments the antitumor efficacy of the AURKA inhibitor MLN8237 on neuroblastoma cells.

ARG51679: IHC-P / Human

Yang Yan et al.
Cancer Cell Int.,  (2020)

publication_link

 

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