CHO Residual DNA (qPCR) Detection Kit
Protocol Booklet
Key features and details
- Standard Curve Linear Range:
- Reference Standard:
- Quantitation Limit:
- Detection Principle:
- Fluorescent Dye:
- Accuracy (Spiked Recovery):
-
Brand:
CAT.NO. : ARK6630
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Size:
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Product Details
Description
As one of the commonly used tool cells, CHO cells are widely applied in the biomedical industry. However, residual host cell DNA poses uncontrollable risks when entering the human body through biological products. Therefore, regulatory agencies such as the WHO, FDA, and China's NMPA have strict limit requirements for the residual amount of host cell DNA to ensure that its nucleic acid residue is within a safe and controllable range. The CHO Residual DNA (qPCR) Detection Kit is highly specific and sensitive, and it uses the qPCR method to quantitatively detect the residual DNA of CHO cells in semi-finished products, intermediates, and finished products of biological products.
Specifications
| Standard Curve Linear Range | 3 fg/μL-300 pg/μL |
|---|---|
| Reference Standard | Traceable to National Reference Standard |
| Detection Principle | Fluorescent Probe qPCR Method |
| Fluorescent Dye | FAM |
| Applicable Instruments | Suitable for Multiple Domestic and Imported Quantitative PCR Instruments |
| Accuracy (Spiked Recovery) | 70%-130% |
| Limit of Quantitation | 0.003 pg/μL, CV≤20%, Measurement Deviation ≤±20% |
| Precision (Repeatability) | For high and medium concentration reference standards, 10 times each, CV<15% |
| Negative Reference Standard Compliance Rate | NTC is Undetermined or Ct value >35 |
Product Features
• High Stability: Less affected by the environment, with stable performance within the shelf life.
• High Precision: CV value of repeatability for low concentration < 20%; CV value of repeatability for medium and high concentrations < 15%.
• Strong Specificity: Not interfered by exogenous DNA from Human, E. coli, and Vero.
• Fast and Convenient: Easy to operate, with results available within 1 hour.
• High Precision: CV value of repeatability for low concentration < 20%; CV value of repeatability for medium and high concentrations < 15%.
• Strong Specificity: Not interfered by exogenous DNA from Human, E. coli, and Vero.
• Fast and Convenient: Easy to operate, with results available within 1 hour.
Typical Data
Storage/Stability
The kit should be stored at -20℃ and has a shelf life of 12 months. Unused portions of the opened kit should be stored under the specified storage conditions. The production date and expiration date can be found on the kit label.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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