claudin-1 Mouse Monoclonal Antibody (XX7)
Key features and details
- Reactivity:Human, Mouse, Rat
- Application:WB, IP, IF, IHC(P), ELISA
- Host:Mouse
- Clonality:Monoclonal
- lsotype:IgG2aK
- Target Name:claudin-1
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Brand:
CAT.NO. : ARA0127
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Product Details
Background
The claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction - bearing tissues. Three classes of proteins are known to localize to tight junctions, including the claudins, Occludin and Junction adhesion molecules. Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is often highly restricted to specific regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin - 1 is a multi - pass membrane protein that is expressed at high levels in kidney and liver and at lower levels in spleen, heart, brain, lung and testis. Defects in the gene encoding claudin - 1 are the cause of ichthyosis - sclerosing cholangitis neonatal syndrome (NISCH), an autosomal recessive syndrome characterized by vulgar type ichthyosis, scalp hypotrichosis, scarring alopecia and sclerosing cholangitis.
Application
claudin - 1 (XX7) is recommended for detection of claudin - 1 of mouse, rat and human origin by Western Blotting (starting dilution 1:200, dilution range 1:100 - 1:1000), immunoprecipitation [1 - 2 µg per 100 - 500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50 - 1:500), immunohistochemistry (including paraffin - embedded sections) (starting dilution 1:50, dilution range 1:50 - 1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30 - 1:3000).
Data
claudin - 1 (XX7). Western blot analysis of claudin - 1 expression in non - transfected 293T (A), human claudin - 1 transfected 293T (B) and SCC - 4 (C) whole cell lysates.
claudin - 1 (XX7). Immunoperoxidase staining of formalin - fixed, paraffin - embedded human stomach tissue showing membrane and cytoplasmic localization (A).
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