Claudin 4 Mouse Monoclonal Antibody(ARA845)
Key features and details
- Reactivity:Human
- Application:WB, IHC
- Host:Mouse
- Clonality:Monoclonal
- Target:Claudin 4
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Brand:
CAT.NO. : ARA6669
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Product Details
Background
Claudin-4 belongs to the 20-member family of claudins, transmembrane cellular adhesion proteins localized at epithelial and endothelial tight junctions. Claudins demonstrate distinct, tissue-specific expression patterns, and are essential components of tight junction charge-specific channels that regulate paracellular ion flux. Claudin-4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl- permeability. In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels. Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines, and in other epithelial cancers. The Claudin-4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems.
Application
|
Application |
Dilution Ratio |
|
WB |
1:500 - 1:1000 |
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IHC |
1:100 - 1:300 |
Overview
|
Item |
Details |
|
Immunogen |
KLH - conjugated synthetic peptide encompassing a sequence within human Claudin 4. The exact sequence is proprietary. |
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Purification Method |
The antibody was purified by immunogen affinity chromatography. |
|
Clone Type |
Monoclonal |
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Product Form |
Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. |
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Gene Name |
CLDN4 |
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Related Names |
CPER; CPETR1; WBSCR8; Claudin - 4; Clostridium perfringens enterotoxin receptor; CPE - R; CPE - receptor; Williams - Beuren syndrome chromosomal region 8 protein |
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Gene ID (Human) |
1364 |
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Protein ID (Human) |
O14493 |
Data
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Immunohistochemical analysis of Claudin 4 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunohistochemical analysis of Claudin 4 staining in human pancreatic carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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