Collagen II Rabbit Polyclonal Antibody
Key features and details
- Reactivity:Human, Mouse
- Predicted Reactivity:Dog, Cow, Pig, Horse, Chicken, Rabbit
- Application:WB, IHC, ICC/IF
- Host:Rabbit
- Clonality:polyclonal
- Target:Collagen II
-
Brand:
CAT.NO. : ARA6480
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Product Details
Background
This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]
Application
|
Application |
Dilution Ratio |
|
WB |
1:300 - 1:5000 |
|
IHC |
1:200 - 1:400 |
|
ICC/IF |
1:50 - 1:200 |
Overview
|
Host |
Rabbit |
|
Source |
KLH conjugated synthetic peptide derived from human Collagen alpha-1(II) chain |
|
Clonality |
Polyclonal |
|
Isotype |
IgG |
|
Entrez Gene |
1280 |
|
Swiss Prot |
P02458 |
|
Purification |
Purified by Protein A. |
|
Storage Buffer |
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
|
Cross Reactive Species |
Human, Mouse |
|
Predicted Cross Reactive Species |
Dog, Cow, Pig, Horse, Chicken, Rabbit |
Data
Lane 1: 293T lysates;
Lane 2: A549 lysates;
Lane 3: LOVO lysates;
Lane 4: MCF-7 lysates;
Lane 5: HepG2 lysatesprobed with Collagen II Rabbit Polyclonal Antibody at 1:300 dilution and 4°C overnight incubation.Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37'C
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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