Connexin 43 Rabbit Monoclonal Antibody(ARA858)

Key features and details

Reactivity:Human
Application:IHC
Host:Rabbit
Clonality:Monoclonal
Target:Connexin 43

Brand:

CAT.NO. : ARA6684

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Size:

1mL

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Product Details

Background

The connexins are a group of gap junction proteins which form a hexamer to compose a connexon. Clusters of connexons form a gap junction through which low molecular weight proteins may diffuse from cell to cell. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and gap junction communication. In Src transformed cells, there is a decrease in gap junctional communication, which appears to be associated with tyrosine phosphorylation of connexin 43. Activated c-Src phosphorylates the C-terminal tail of connexin 43 on Tyr 265, resulting in a stable interaction between both proteins, which leads to inhibition of gap junctional communication. In addition to tyrosine phosphorylation, connexin 43 has also been shown to be phosphorylated on serine in the absence of Src kinases and on both serine and tyrosine in cells expressing Src kinases, such as c-Src and/or pp60v-Src. In human vascular endothelial cells, connexin 43 is posttranslationally modified during mitosis. Mitosis-specific phosphorylation of connexin 43 correlates with the transient loss of gap junction intercellular communication and redistribution of connexin 43.

Application

Application	Dilution Ratio
IHC-P	1:2,000-1:5,000

Overview

Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Recombinant protein within human Connexin 43 aa 219 - 382
Species Reactivity	Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Molecular Weight	Predicted band size: 43 kDa
Positive Control	A549 cell lysate, PC-12 cell lysate, mouse heart lysate, human heart tissue, human testis tissue
Conjugation	unconjugated
RRID	AB_3072429
Form	Liquid
Concentration	1ug/ul
Storage Buffer	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide
Isotype	IgG
Purification Method	Protein A affinity purified