Cyclin D1 Rabbit Monoclonal Antibody(ARB932)
Key features and details
- Target:
- Clone ID:
- Host:
- Molecular Weight:
- Purity:
- Species Cross-reactivity:
- Applications:
- Swissprot ID:
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Brand:
CAT.NO. : ARB6724
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Size:
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Product Details
Background
Cyclin D1 (synonymes: PRAD1, parathyroid adenomatosis 1, CCND1) is a 295 amino acid protein, 36 kDa, which belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The protein has been shown to interact with tumour suppressor protein Rb and its expression is regulated positively by Rb. The expression is cell cycle dependant, maximal in G1 and minimal in S phase. Localization is mainly nuclear. In normal tissues, Cyclin D1 expression is restricted to the proliferative zone of epithelial tissues, endothelium and some fibroblasts. There is no expression in lymphoid tissue. Mutations with amplification of the cyclin D1 gene, with overexpression of the protein, which alters cell cycle progression, are observed frequently in a variety of tumours and may contribute to tumourigenesis. In diagnostic pathology, immunohistochemical detection of Cyclin D1 is used mainly for the diagnosis of mantle cell lymphoma. While other methods of cyclin D1 detection are being explored, immunohistochemistry is still widely used and anti-cyclin D1 antibodies remain an essential component in a small B-cell lymphoproliferative disorder panel.
Application
| Application | Dilution Ratio |
|---|---|
| IHC | 1:100-1:200 |
Overview
| Predicted Molecular Wt | 34kDa |
| Species Cross-reactivity | Human |
| Applications | HIC-P |
| Purity | ProA affinity purified IgG |
| Form | Liquid |
| Swissprot ID | P24385 |
| Subcellular location | Nucleus |
| Recommended method | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. |
| Immunogen | Synthetic peptide corresponding to residues within aa200-295 of Cyclin D1 |
| Storage Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. |
Data
Immunohistochemical staining of human mantle cell lymphoma tissue using Cyclin D1 Rabbit Monoclonal Antibody (ARB932).
Storage
Store at -20°C. Stable for one year from the date of shipment.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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