Cyclin E1 Rabbit Monoclonal Antibody(ARA952)

Key features and details

Reactivity:Human
Application:IHC
Host:Rabbit
Clonality:Monoclonal
Target:Cyclin E1

Brand:

CAT.NO. : ARA6794

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Size:

1mL

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Product Details

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.

Application

Application	Dilution Ratio
IHC-P	1:200-1:1000

Overview

Antibody Type	Recombinant Rabbit Monoclonal Antibody
Immunogen	Synthetic peptide within Human Cyclin E1 aa 361 - 410 / 410
Species Reactivity	Human, Mouse
Validated Applications	WB, IF-Cell, IF-Tissue, IHC-P
Molecular Weight	Predicted band size: 47 kDa
Positive Control	HeLa cell lysate, Human lung tissue lysate, Human kidney tissue lysate, HeLa, human placenta tissue, human colon carcinoma tissue, mouse testis tissue
Conjugation	unconjugated
Clone Number	SD20-24
Form	Liquid
Concentration	1μg/ul
Storage Buffer	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide
Isotype	IgG
Purification Method	Protein A affinity purified