Cytokeratin 1 Rabbit Monoclonal Antibody(ARB933)
CAT.NO. : ARB6725
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Background
In normal tissues, cytokeratin (high molecular weight) is mainly expressed in normal squamous epithelium, ductal epithelium and other laminated epithelium. Positive expression was found in lung squamous cell carcinoma, breast cancer, pancreatic cancer, cholangiocarcinoma and bladder transitional cell carcinoma. Anti-cytokeratin, high molecular weight is capable of recognizing all basic keratins; therefore, it is a broadly reactive antibody staining most epithelia and their neoplasms. Members of the acidic and basic subfamilies are found together in pairs. Since each epithelium contains at least one acidic and one basic keratin, this antibody is used to observe the distribution of keratin-containing cells in normal epithelia and to identify neoplasms derived from such epithelium.
Application
Application | Dilution Ratio |
---|---|
IHC | 1:100-1:200 |
Overview
Purity | ProA affinity purified IgG |
Form | Liquid |
Species Cross-reactivity | Human |
Applications | IHC-P |
Subcellular location | Cytoplasm |
Recommended method | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. |
Immunogen | Synthetic peptide corresponding to residues within aa200-300 of Cytokeratin 1 and aa490-590 (C-terminal) of Cytokeratin 5 |
Storage Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. |
Data

Immunohistochemical staining of human esophagus tissue using Cytokeratin 1 Rabbit Monoclonal Antibody (ARB933).
Storage
Store at -20°C. Stable for one year from the date of shipment.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
