EGFR Rabbit Monoclonal Antibody(ARA715)
Key features and details
- Reactivity:Human, Mouse, Rat, Monkey
- Application:WB, IF/ICC, IP, FC
- Host:Rabbit
- Clonality:Monoclonal
- lsotype: IgG
- Target:EGFR
-
Brand:
CAT.NO. : ARA6488
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Product Details
Background
Phosphorylated on Tyr residues in response to EGF. Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.; Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126 (By similarity).; Palmitoylated on Cys residues by ZDHHC20. Palmitoylation inhibits internalization after ligand binding, and increases the persistence of tyrosine-phosphorylated EGFR at the cell membrane. Palmitoylation increases the amplitude and duration of EGFR signaling.; Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
Application
|
Application |
Dilution Ratio |
|
WB |
1:500 - 1:20,000 |
|
IF/ICC |
1:100 - 1:500 |
|
FC |
1:100 - 1:1000 |
|
IP |
|
Overview
|
Antibody Type |
Recombinant Rabbit monoclonal Antibody |
|
Immunogen |
Synthetic peptide within human EGFR aa 1070-1110. |
|
Species Reactivity |
Human, Mouse, Rat, Monkey |
|
Validated Applications |
WB, IF/ICC, IP, FC |
|
Molecular Weight |
Predicted band size: 134 kDa |
|
Positive Control |
HeLa cell lysate, A431 cell lysate, MDA-MB-468 cell lysate, A431, Mouse placenta tissue lysate, Mouse lung tissue lysate, Rat skin tissue lysate, Rat liver tissue lysate. |
|
Conjugation |
unconjugated |
|
RRID |
AB_3069699 |
|
Form |
Liquid |
|
Storage Buffer |
1×TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
|
Isotype |
IgG |
Data
Western blot analysis of EGFR with EGFR Rabbit Monoclonal Antibody(ARA715).
Immunocytochemistry analysis of A431 cells labeling EGFR with EGFR Rabbit Monoclonal Antibody(ARA715).
Flow cytometric analysis of A431 cells labeling EGFR. Cells were fixed and permeabilized. Then stained with the EGFR Rabbit Monoclonal Antibody(ARA715) (red) at 1/1,000 dilution.
EGFR was immunoprecipitated from 0.2 mg HeLa cell lysate with EGFR Rabbit Monoclonal Antibody(ARA715) at 2 µg/10 µl beads.
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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