Fibrinogen alpha chain Rabbit Monoclonal Antibody (ARA822)
Key features and details
- Reactivity:Human, Mouse, Rat
- Application:WB, IHC(P)
- Host:Rabbit
- Clonality:Monoclonal
- lsotype:IgG
- Target:Fibrinogen alpha chain
-
Brand:
CAT.NO. : ARA6614
US$ Please choose
US$ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。
Product Details
Background
Fibrinogen alpha chain is a protein that in humans is encoded by the FGA gene. The protein encoded by this gene is the alpha component of fibrinogen, a blood-borne glycoprotein composed of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin, which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia, and renal amyloidosis. Alternative splicing results in two isoforms that vary in the carboxy-terminus.
Application
|
Applications |
Tested Dilution |
|
WB |
1:1,000 |
|
IHC-P |
1:500-1:1,000 |
Overview
|
Molecular Weight |
Predicted band size: 95 kDa |
|
Clonality |
Monoclonal |
|
Species Reactivity |
Human, Mouse, Rat |
|
Isotype |
IgG |
|
Host |
Rabbit |
|
Concentration |
1ug/ul |
|
Form/Format |
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
|
Purity/Purification |
Purified |
|
Immunogen |
Synthetic peptide. |
Data
Western blot analysis of Fibrinogen alpha chain on different lysates with Fibrinogen alpha chain Rabbit Monoclonal Antibody (ARA822) at 1/1,000 dilution.
Lane 1: Human plasma lysate (30 µg/Lane)
Lane 2: Mouse plasma lysate (30 µg/Lane)
Lane 3: Rat plasma lysate (30 µg/Lane)
Predicted band size: 95 kDa
Observed band size: 100 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ARA822) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta labeling Fibrinogen alpha chain with ARA822.
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
New Products
