FITC Conjugated Goat IgG Rabbit Polyclonal Antibody
Key features and details
- Reactivity:Goat
- Application:IF/ICC, FC
- Host:Rabbit
- Clonality:Polyclonal
- Target:Goat IgG
-
Brand:
CAT.NO. : ARA6500
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Size:
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Product Details
Background
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. FITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all of our antibodies and purified proteins, with the exception of streptavidin. Fluorescein conjugates absorb light maximally at 492 nm and fluoresce maximally at 520 nm. Although less bright than other green-fluorescing dyes, FITC is still a widely used fluorophore due to its long history. The major disadvantage of fluorescein is its rapid photobleaching (fading), which can be mitigated by the use of an anti-fading agent in the mounting medium. A better choice for many applications involving FITC is Alexa Fluor® 488 because it is brighter and more photostable.
Application
|
Application |
Dilution Ratio |
|
IF/ICC |
1:100 - 1:1000 |
|
FC |
1:100 - 1:1000 |
Overview
|
Antibody Type |
Rabbit Polyclonal Antibody |
|
Immunogen |
Goat IgG |
|
Species Reactivity |
Goat |
|
Validated Applications |
IF/ICC, FC |
|
Conjugation |
FITC |
|
Form |
Liquid |
|
Storage Buffer |
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS |
|
Isotype |
IgG |
|
Purification Method |
Immunogen affinity purified. |
Data
Immunocytochemistry analysis of HeLa cells labeling beta Actin with Mouse beta Actin antibody. FITC Conjugated Goat IgG Rabbit Polyclonal Antibody was used as the secondary antibody at 1/1,000 dilution.
Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling CD34 with Mouse anti-CD34 antibody.FITC Conjugated Goat IgG Rabbit Polyclonal Antibody was used as the secondary antibody at 1/1,000 dilution.
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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