FPN1/Ferroportin/SLC40A1 Rabbit Polyclonal Antibody
Key features and details
- Reactivity:Human, Mouse, Rat
- Application:WB, IHC, IF, FCM, ELISA
- Host:Rabbit
- Clonality:Polyclonal
- lsotype:IgG
- Target:FPN1/Ferroportin/SLC40A1
-
Brand:
CAT.NO. : ARA6618
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Product Details
Background
Ferroportin (FPN1 or SLC40A1) is the primary protein responsible for iron export from cells in mammals. It transports iron from the inside of a cell to the outside, and is essential for maintaining iron balance in the body. Disruptions in FPN1 function can lead to iron overload or deficiency.
Application
|
Applications |
Tested Dilution |
|
Western Blot (WB) |
1:500-2000 |
|
Immunohistochemistry (IHC) |
1:50-400 |
|
Immunocytochemistry (ICC/IF) |
1:50-400 |
|
Flow Cytometry (Flow) |
1-3 μg/1x106 cells |
|
Suggested positive control: human intestine protein. |
|
Overview
|
Species Reactivity |
Human, Mouse, Rat |
|
Application |
WB, IHC, IF, FCM, ELISA |
|
Host/Isotype |
Rabbit / IgG |
|
Class |
Polyclonal |
|
Type |
Antibody |
|
Conjugate |
Unconjugated |
|
Immunogen |
Peptide (C)EGTHLMGVKDSNIHE, corresponding to amino acid residues 261-275 of human Ferroportin . 2nd intracellular loop. Note: the structure of Ferroportin is still controversial and there is no defined topology yet. |
|
Form |
Liquid |
|
Concentration |
1 mg/mL |
|
Purification |
Antigen affinity chromatography |
|
Storage buffer |
PBS, pH 7.4, 1% BSA. |
|
Contains |
0.05% sodium azide |
Data
Western blot analysis of rat spleen (lanes 1 and 5), rat small intestine (lanes 2 and 6), rat liver (lanes 3 and 7) and mouse liver (lanes 4 and 8) lysates.
1-4. Anti-Ferroportin (SLC40A1) Antibody (#ARA6618), (1:500). 5-8. Anti-Ferroportin (SLC40A1) Antibody, preincubated with Ferroportin/SLC40A1 Blocking Peptide .
Immunohistochemical staining of perfusion-fixed frozen mouse substantia nigra pars compacta (SNC) sections using Anti- Ferroportin (SLC40A1) Antibody (#ARA6618). A. Ferroportin staining (green) appears in several cells in the SNC. B. Tyrosine hydroxylase (red) stains dopaminergic neurons. C. Merge of A and B demonstrates Ferroportin in dopaminergic and non- dopaminergic neurons. Cell nuclei are stained using DAPI (blue).
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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