GAP43 Rabbit Monoclonal Antibody(ARA867)

Key features and details

Reactivity:Human
Application:IHC
Host:Rabbit
Clonality:Monoclonal
Target:CD47

Brand:

CAT.NO. : ARA6693

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Size:

1mL

Trail, Bulk size or Custom requests Please contact us

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Product Details

Background

GAP43, is a nervous tissue-specific cytoplasmic protein that can be attached to the membrane via a dual palmitoylation sequence on cysteines 3 and 4. This sequence targets GAP43 to lipid rafts. It is a major protein kinase C (PKC) substrate and is considered to play a key role in neurite formation, regeneration, and plasticity. The role of GAP-43 in CNS development is not limited to effects on axons: It is also a component of the centrosome, and differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. GAP43, the consensus choice for its designation, is a nervous system-specific protein that is attached to the membrane via a dual palmitoylation sequence on cysteines 3 and 4, though it can exist in the non-bound form in the cytoplasm. This dual sequence enables the association of phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] or PIP2, with actin, facilitating the latter's polymerization thereby regulating neuronal structure. GAP-43 is also a protein kinase C (PKC) substrate. Phosphorylation of serine-41 on GAP-43 by PKC regulates neurite formation, regeneration, and synaptic plasticity.

Application

Application	Dilution Ratio
IHC-P	1:10000-1:15000

Overview

Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Synthetic peptide within Human GAP43 aa 189-238 / 238
Species Reactivity	Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Validated Applications	WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Molecular Weight	Predicted band size: 25 kDa
Positive Control	SH-SY5Y cell lysate, Neuro-2a cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human glioma tissue, Neuro-2a, rat hippocampus tissue lysate, mouse hippocampus tissue lysate, mouse brain tissue, rat brain tissue, rat hippocampus tissue, SH-SY5Y
Conjugation	unconjugated
RRID	AB_3089981
Form	Liquid
Concentration	1μg/ul
Storage Buffer	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol, Preservative: 0.05% Sodium Azide
Isotype	IgG
Purification Method	Protein A affinity purified

Data

Immunohistochemical analysis of paraffin-embedded human glioma tissue with GAP43 Rabbit Monoclonal Antibody(ARA867)

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Research Use Only

For Research Use Only. Not for use in diagnostic procedures.

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