GFP tag Mouse Monoclonal antibody(ARA705)
Key features and details
- Reactivity:Species independent
- Application:WB, IP, ICC/IF
- Host:Mouse
- Clonality:Monoclonal
- lsotype: IgG2a
- Target:GFP tag
-
Brand:
CAT.NO. : ARA6477
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Product Details
Background
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. It has been used in modified forms to make biosensors, and many animals have been created that express GFP, which demonstrates a proof of concept that a gene can be expressed throughout a given organism, in selected organs, or in cells of interest. GFP can be introduced into animals or other species through transgenic techniques, and maintained in their genome and that of their offspring. To date, GFP has been expressed in many species, including bacteria, yeasts, fungi, fish and mammals, including in human cells.
Application
|
Application |
Dilution Ratio |
|
WB |
1:10000-1:100000 |
|
IP |
0.5-5.0 ug for 1.0-5.0 mg of total protein lysate |
|
IF/ICC |
1:500-1:1500 |
Overview
|
Reactivity |
Species independent |
|
Host / Isotype |
Mouse / IgG2a |
|
Class |
Monoclonal |
|
Type |
Antibody |
|
Immunogen |
Recombinant aequorea victoria GFP tag protein |
|
Full Name |
GFP tag |
|
Calculated Molecular Weight |
26 kDa |
|
GenBank Accession Number |
M62653 |
|
RRID |
AB_11182611 |
|
Conjugate |
Unconjugated |
|
Form |
Liquid |
|
Purification Method |
Protein A purification |
|
UNIPROT ID |
P42212 |
|
Storage Buffer |
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Data
Recombinant protein were subjected to SDS PAGE followed by western blot with GFP tag Mouse Monoclonal antibody(ARA705) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP result of GFP tag Mouse Monoclonal antibody(ARA705) (IP:4ug; Detection:1:10000) with Transfected HEK-293T cells lysate 400 ug.
Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using GFP tag Mouse Monoclonal antibody(ARA705) at dilution of 1:800 and Fluor 594-Conjugated AffiniPure Mouse IgG(H+L) Goat antibody.
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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