GFP tag Rabbit Monoclonal antibody(ARA704)

Key features and details

  • Reactivity:Species independent
  • Application:WB, IF/ICC
  • Host:Rabbit
  • Clonality:Monoclonal
  • lsotype: IgG1
  • Target:GFP tag
  • Brand:
CAT.NO. : ARA6465
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Size:
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Product Details
Background
Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm. When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. This antibody is generated against the full-length eGFP protein. It exhibits reactivity towards variants of Aequorea victoria GFP, including S65T-GFP, RS-GFP, YFP, CFP, and eGFP.
Application

Application

Dilution Ratio

WB

1:5000 - 1:40000

IF/ICC

1:200 - 1:10000

Overview

Host / Isotype

Rabbit / IgG

Class

Recombinant

Type

Antibody

Immunogen

Recombinant aequorea victoria GFP tag protein

Full Name

GFP tag

Calculated Molecular Weight

26 kDa

GenBank Accession Number

M62653

RRID

AB_3670509

Conjugate

Unconjugated

Form

Liquid

Purification Method

Protein A purification

UNIPROT ID

P42212

Storage Buffer

PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Data
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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