Gli1 Rabbit Monoclonal Antibody(ARA885)

Key features and details

Reactivity:Human, Mouse, Rat
Application:IHC
Host:Rabbit
Clonality:Monoclonal
Target:Gli1

Brand:

CAT.NO. : ARA6712

RMB Please choose

Size:

1mL

Trail, Bulk size or Custom requests Please contact us

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

Background

Zinc finger protein GLI1 also known as glioma-associated oncogene is a protein that in humans is encoded by the GLI1 gene. It was originally isolated from human glioblastoma cells. The Gli proteins are the effectors of Hedgehog (Hh) signaling and have been shown to be involved in cell fate determination, proliferation and patterning in many cell types and most organs during embryo development. In the developing spinal cord the target genes of Gli proteins, that are themselves transcription factors, are arranged into a complex gene regulatory network that translates the extracellular concentration gradient of Sonic hedgehog into different cell fates along the dorsoventral axis. The Gli transcription factors activate/inhibit transcription by binding to Gli responsive genes and by interacting with the transcription complex. The Gli transcription factors have DNA binding zinc finger domains which bind to consensus sequences on their target genes to initiate or suppress transcription.

Application

Application	Dilution Ratio
IHC-P	1:50-1:200

Overview

Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Synthetic peptide within Human Gli1 aa 38-82 / 1106
Species Reactivity	Human, Mouse, Rat
Validated Applications	WB, IHC-P, IF-Cell
Molecular Weight	Predicted band size: 118 kDa
Positive Control	K-562 cell lysate, HeLa cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, HeLa, Neuro-2a, C6, mouse fallopian tissue, rat testis tissue
Conjugation	unconjugated
RRID	AB_3070345
Form	Liquid
Concentration	1μg/ul
Storage Buffer	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol, Preservative: 0.05% Sodium Azide
Isotype	IgG
Purification Method	Protein A affinity purified