Glucose 6 phosphate Dehydrogenase Activity Assay Kit (Colorimetric)
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- 反应物种:
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- 偶联标记说明:
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CAT.NO. : ARG82165
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Product Details
概述
| 产品描述 | ARG82165 Glucose 6 phosphate Dehydrogenase Activity Assay Kit (Colorimetric) can be used to measure Glucose 6 phosphate Dehydrogenase activity in serum, plasma, tissue and cell culture supernatants. |
|---|---|
| 反应物种 | Hu, Ms, Rat, All |
| 应用 | FuncSt |
| 靶点名称 | Glucose 6 phosphate Dehydrogenase |
| 偶联标记说明 | Read at 460 nm |
| 灵敏度 | 0.2 U/L |
| 检测范围 | 0.2 - 100 U/L |
| 样品类型 | serum, plasma, tissue and cell culture supernatants |
| 样本量 | 20 µl |
| 別名 | G6PD1; G6PD; EC 1.1.1.49; Glucose-6-phosphate 1-dehydrogenase |
应用说明
| 应用说明 | Please note that this kit does not include a microplate. |
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| 检测时间 | 20 min |
属性
| 形式 | Liquid |
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| 存放说明 | Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P05370 Rat Glucose-6-phosphate 1-dehydrogenase Swiss-port # P11413 Human Glucose-6-phosphate 1-dehydrogenase |
|---|---|
| 基因名称 | G6PD |
| 全名 | glucose-6-phosphate dehydrogenase |
| 背景介绍 | This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. [UniProt] |
| 翻译后修饰 | Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity. [UniProt] |
检测图片 (1)
ARG82165 Glucose 6 phosphate Dehydrogenase Activity Assay Kit (Colorimetric) enzyme kinetics graph
Kinetics of 0 and 100 U/L G6PDH reaction, using ARG82165 Glucose 6 phosphate Dehydrogenase Activity Assay Kit (Colorimetric).
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