Glutaminase Assay Kit (Colorimetric)
Key features and details
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- 反应物种:
- 应用:
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- 靶点名称:
- 偶联标记说明:
- 检测范围:
- 样品类型:
- 样本量:
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Brand:
CAT.NO. : ARG82767
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Product Details
概述
| 产品描述 | ARG82767 Glutaminase Assay Kit (Colorimetric) is a detection kit for the quantification of Glutaminase activity in cells, bacteria and tissue samples. |
|---|---|
| 反应物种 | All |
| 应用 | FuncSt |
| 特异性 | The assay is initiated with the enzymatic hydrolysis of Glutamine by Glutaminase. The enzyme catalysed reaction products can be measured at a colorimetric readout at 620 nm. |
| 靶点名称 | Glutaminase |
| 偶联标记说明 | Read at 620 nm (colorimetric) |
| 检测范围 | 0.01 - 1 μmol/ml |
| 样品类型 | Cells, bacteria and tissue samples. |
| 样本量 | 20 µl |
| 別名 | EC 3.5.1.2; AAD20; L-glutamine amidohydrolase; KGA; Glutaminase kidney isoform, mitochondrial; GAM; K-glutaminase; GLS; GAC; GLS1 |
应用说明
| 检测时间 | ~ 1.5 hours |
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属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | GLS |
|---|---|
| 全名 | glutaminase |
| 背景介绍 | This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012] |
| 生物功能 | Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain (PubMed:30575854, PubMed:30239721, PubMed:30970188). [Isoform 2]: Lacks catalytic activity. [UniProt] |
| 细胞定位 | Isoform 1: Cytoplasm, cytosol. Isoform 3: Mitochondrion. [UniProt] |
| 产品亮点 | Related products: Glutaminase antibodies; Glutaminase ELISA Kits; Related news: TCA intermediate fumarate promotes mitobiogenesis |
检测图片 (1)
ARG82767 Glutaminase Assay Kit (Colorimetric) typical data demonstration image
ARG82767 Glutaminase Assay Kit (Colorimetric) results of a typical standard run with optical density reading at 620 nm, using colorimetric reading.
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