Glutathione Peroxidase Assay Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记说明:
- 灵敏度:
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- 样品类型:
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Brand:
CAT.NO. : ARG82117
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Product Details
内含物
| 货号 | 内含物名称 | 包装 |
|---|---|---|
| ARG82117-001 | Assay Buffer | 25 ml |
| ARG82117-002 | GR Enzyme | 250 μl |
| ARG82117-003 | Glutathione | 1 vial (lyophilized) |
| ARG82117-004 | NADPH | 1 vial (lyophilized) |
| ARG82117-005 | Standard | 1 vial (lyophilized) |
| ARG82117-006 | Peroxide Solution | 50 μl |
概述
| 产品描述 | ARG82117 Glutathione Peroxidase Assay Kit is a detection kit for the quantification of Glutathione Peroxidase activity in serum, plasma, tissue and cell lysates. |
|---|---|
| 反应物种 | All |
| 应用 | FuncSt |
| 靶点名称 | Glutathione Peroxidase |
| 偶联标记说明 | Read at 340 nm. |
| 灵敏度 | 40 U/l |
| 检测范围 | 40 - 800 U/l |
| 样品类型 | Serum, plasma, tissue and cell lysates. |
| 样本量 | 10 µl |
| 別名 | GPXD; Glutathione peroxidase 1; GPx-1; Cellular glutathione peroxidase; EC 1.11.1.9; GSHPX1; GSHPx-1 |
应用说明
| 应用说明 | Note: Please note that this kit does not include a microplate. |
|---|---|
| 检测时间 | 20 min |
属性
| 形式 | Liquid |
|---|---|
| 存放说明 | Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | GPX1 |
|---|---|
| 全名 | glutathione peroxidase 1 |
| 背景介绍 | This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Protects the hemoglobin in erythrocytes from oxidative breakdown. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 翻译后修饰 | During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine. [UniProt] |
检测图片 (1)
ARG82117 Glutathione Peroxidase Assay Kit standard curve image
ARG82117 Glutathione Peroxidase Assay Kit results of a typical standard run with optical density reading at 340 nm.
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