GPNMB Rabbit Monoclonal Antibody(ARA886)
Key features and details
- Reactivity:Human, Mouse, Rat
- Application:IHC
- Host:Rabbit
- Clonality:Monoclonal
- Target:GPNMB
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Brand:
CAT.NO. : ARA6713
RMB Please choose
RMB Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
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Product Details
Background
Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. Two transcript variants encoding 560 and 572 amino acid isoforms have been characterized for this gene in humans. The mouse and rat orthologues of GPNMB are known as DC-HIL and Osteoactivin (OA), respectively. GPNMB is a type I transmembrane glycoprotein which shows homology to the pmel17 precursor, a melanocyte-specific protein. GPNMB has been reported to be expressed in various cell types, including: melanocytes, osteoclasts, osteoblasts, dendritic cells, and it is overexpressed in various cancer types. In melanocytic cells and osteoclasts the GPNMB gene is transcriptionally regulated by Microphthalmia-associated transcription factor. In osteoblast progenitor cells, Osteoactivin works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization[9] that is mediated at least in part by Bone_morphogenetic_protein_2 in a SMAD1 dependent manner to promote osteoblast differentiation. In addition, using a rat fracture model, Osteoactivin (OA) enhances the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis (soft callus) and osteogenesis (hard callus) compared to the intact femurs that is why Osteoactivin (OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, Osteoactivin expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.
Application
|
Application |
Dilution Ratio |
|
IHC-P |
1:100-1：200 |
Overview
|
Antibody Type |
Recombinant Rabbit monoclonal Antibody |
|
Immunogen |
Recombinant protein within human GPNMB aa 1-498 / 572 |
|
Species Reactivity |
Human, Mouse, Rat |
|
Validated Applications |
WB, IHC-P, IF-Cell, FC |
|
Molecular Weight |
Predicted band size: 64 kDa |
|
Positive Control |
L929 cell lysate, A375 cell lysate, U-937 cell lysate, rat heart tissue lysate, BL6F1 cell lysates, human lung tissue, L929 |
|
Conjugation |
unconjugated |
|
RRID |
AB_3072627 |
|
Form |
Liquid |
|
Concentration |
1μg/ul |
|
Storage Buffer |
PBS (pH7.4), 0.1% BSA, 40% Glycerol, Preservative: 0.05% Sodium Azide |
|
Isotype |
IgG |
|
Purification Method |
Protein A affinity purified |
Data
Immunohistochemical analysis of paraffin-embedded human lung tissue with GPNMB Rabbit Monoclonal Antibody(ARA886)
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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