GST tag Rabbit Monoclonal Antibody(ARA804)
Key features and details
- Reactivity:Species independent
- Application:WB, IF/ICC, FC, IP
- Host:Rabbit
- Clonality:Monoclonal
- Target:GST tag
-
Brand:
CAT.NO. : ARA6591
RMB Please choose
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Size:
Trail, Bulk size or Custom requests Please contact us
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Product Details
Background
GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.
Application
|
Application |
Dilution Ratio |
|
WB |
1:2,000 - 1:5,000 |
|
IF/ICC |
1:10,000 - 1:40,000 |
|
FC |
1:800 - 1:1,200 |
|
IP |
1:40 - 1:50 |
Overview
|
Predicted Molecular Weight |
Depending on customers' target of interest |
|
Purity |
ProA affinity purified IgG |
|
Species Cross-reactivity |
Species independent |
|
Form |
Liquid |
|
Applications |
WB, IF/ICC, FC, IP |
|
Swissprot ID |
P08515 |
|
Immunogen |
Recombinant full length protein within Schistosoma japonicum GST aa 1-218. The exact sequence is proprietary. |
|
Storage Buffer |
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. |
Data
Predicted MW: Depend on fusion protein with GST tag
Lane 1: 293 cell lysates transfected with N-terminal GST tagged gene (1:100,000 dilution).
Lane 2: 293 cell lysates transfected with C-terminal GST tagged gene (1:20,000 dilution).
Lane 3: two fusion proteins, one (45KD) with GST tag on C-terminal (1:2,000 dilution), the other (83KD) with GST tag on N-terminal (1:2,000 dilution).
Lane 4: Mock 293 cell lysates (1:10,000 dilution)
Lane 1&2: 2 μg per lane
Lane 3: 20 ng per lane
Lane 4: 10 μg per lane 2nd Ab:
GAR HRP(H+L) 1:5,000
Exposure: 30s
Overlay histogram showing 293 cells transfected with C-terminal (Red) and N terminal (Blue) GST tagged gene stained with GST tag Rabbit Monoclonal Antibody(ARA804).
GST tag Rabbit Monoclonal Antibody(ARA804) staining GST tag in 293 cells transfected with N-terminal GSTtagged gene by IF/ICC (immunofluorescence/immunocytochemistry).
GST tag Rabbit Monoclonal Antibody(ARA804) staining GST tag in 293 cells transfected with C-terminal GSTtagged gene by IF/ICC (immunofluorescence/immunocytochemistry).
GST tag was immunoprecipitated from O.2mg of 293 whole celllysates transfected with C-terminal GST tagged gene with GST tag Rabbit Monoclonal Antibody(ARA804).
GST tag was immunoprecipitated from 0.2mg of 293 whole celllysates transfected with N-terminal GST tagged gene with GST tag Rabbit Monoclonal Antibody(ARA804).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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