HA tag Rabbit Monoclonal Antibody(ARA782)
Key features and details
- Target:
- Clone ID:
- Host:
- Clone Type:
- Purity:
- Species Cross-reactivity:
- Applications:
- Isotype:
-
Brand:
CAT.NO. : ARA6569
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Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This antibody can detect either N-term or C-term HA tag.
Application
|
Application |
Dilution Ratio |
|
WB |
1:400-1:2,000 |
|
IF/ICC |
1:5-1:10 |
|
FC |
1:1,000-1:2,000 |
|
IP |
1:400-1:2,000 |
Overview
|
Host Species |
Rabbit |
|
Species Cross-reactivity |
Species independent |
|
Application |
WB, IF/ICC, FC, IP |
|
Immunogen |
YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to |
|
Purity |
ProA affinity purified IgG |
|
Clone Type |
Recombinant Monoclonal |
|
Isotype |
IgG |
|
Buffer |
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA |
Data
Predicted MW: Depend on fusion protein with HA tag
Lane 1: 293 cells lysate transfected with C-terminal HA tagged gene (HA tag Rabbit Monoclonal Antibody(ARA782) at 1:2,000 dilution).
Lane 2: 293 cells lysate transfected with N-terminal HA tagged gene (HA tag Rabbit Monoclonal Antibody(ARA782) at 1:1,000 dilution).
Lane 3: 293 cells lysate without any transfection (HA tag Rabbit Monoclonal Antibody(ARA782) at 1:400 dilution).
Lane 1: 1 μg per lane
Lane 2/3: 10 μg per lane
2nd Ab:
GAR HRP(H+L) 1:5,000
Exposure: 20s
Overlay histogram showing 293 cells transfected with N-terminal (Green) and C-terminal (Red) HA tagged gene stained with HA tag Rabbit Monoclonal Antibody(ARA782). The cells were then incubated in the antibody (HA tag Rabbit Monoclonal Antibody(ARA782), 1:2,000 dilution) in 1x PBS/1% BSA for 30 min at at room temperature. The secondary antibody used was a Goat Anti-Rabbit AREX® Fluor 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
HA tag Rabbit Monoclonal Antibody(ARA782) staining HA tag in 293 cells transfected with N-terminal HA tagged gene by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:2,000) at 4°C. An AREX® Fluor 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An AREX® Fluor 594-conjugated Goat Anti-Rabbit IgG (1:500).
HA tag Rabbit Monoclonal Antibody(ARA782) staining HA tag in 293 cells transfected with C-terminal HA tagged gene by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:2,000) at 4°C. An AREX® Fluor 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An AREX® Fluor 594-conjugated Goat Anti-Rabbit IgG (1:500).
HA tag was immunoprecipitated from 0.2mg of 293 whole cells lysate transfected with N-terminal HA tagged gene with HA tag Rabbit Monoclonal Antibody(ARA782) at 1:10 dilution.
2nd Ab:
GAR HRP for IP 1:500
Lane 1: HA tag Rabbit Monoclonal Antibody(ARA782) IP in 293 whole cell lysate transfected with N-terminal HA tagged gene
Lane 2: PBS instead of HA tag Rabbit Monoclonal Antibody(ARA782) in 293 whole cell lysate transfected with N-terminal HA tagged gene
Lane 3: 293 whole cell lysate transfected with N-terminal HA tagged gene, 10 μg (input)
HA tag was immunoprecipitated from 0.2mg of 293 whole cells lysate transfected with C-terminal HA tagged gene with HA tag Rabbit Monoclonal Antibody(ARA782) at 1:10 dilution.
2nd Ab:
GAR HRP for IP 1:500
Lane 1: HA tag Rabbit Monoclonal Antibody(ARA782) IP in 293 whole cell lysate transfected with C-terminal HA tagged gene
Lane 2: PBS instead of HA tag Rabbit Monoclonal Antibody(ARA782) in 293 whole cell lysate transfected with C-terminal HA tagged gene
Lane 3: 293 whole cell lysate transfected with C-terminal HA tagged gene, 10 μg (input)
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Note
For Research Use Only. Not for use in diagnostic procedures.
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