HA tag Rabbit Monoclonal Antibody(ARB574)
Key features and details
- Target:
- Clone ID:
- Host:
- Molecular Weight:
- Purity:
- Species Cross-reactivity:
- Applications:
- Swissprot ID:
-
Brand:
CAT.NO. : ARB6866
RMB Please choose
RMB Please choose
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Product Details
Background
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag derived from the HA molecule corresponding to amino acids 98 - 106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This antibody can detect either N - term or C - term HA tag.
Application
|
Application |
Recommended Dilutions |
|
WB |
1:1000 - 1:2000 |
|
IHC |
1:100 - 1:300 |
|
IF/ICC |
1:400 - 1:2000 |
|
FC |
1:400 - 1:2000 |
|
IP |
1:10 |
Overview
|
Predicted Molecular Wt |
Depending on customers’ target of interest |
|
Species Cross-reactivity |
Species independent |
|
Species cross-reactivity determined by WB |
Species independent |
|
Applications |
WB, IF/ICC, FC, IP |
|
Purity |
ProA affinity purified IgG |
|
Form |
Liquid |
|
Swissprot ID |
N/A |
|
Immunogen |
YPYDVPDYA (Influenza hemagglutinin-HA-epitope) conjugated to KLH |
|
Storage Buffer |
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
Data
Predicted MW: Depend on fusion protein with HA Tag Lane 1: 293 cells lysate transfected with C-terminal HA tagged gene (at 1:2,000 dilution). Lane 2: 293 cells lysate transfected with N-terminal HA tagged gene (at 1:1,000 dilution). Lane 3: 293 cells lysate without any transfection (at 1:400 dilution). Lane 1: 1 μg per lane Lane 2/3: 10 μg per lane 2nd Ab: GAR HRP(H+L) 1:5,000
Overlay histogram showing 293 cells transfected with N-terminal (Green) and C-terminal (Red) HA tagged gene stained with ARB574. The cells were then incubated in the antibody (ARB574, 1:2,000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
ARB574 staining HA tag in 293 cells transfected with N-terminal HA tagged gene by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:2,000) at 4°C. An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG (1:500).
ARB574 staining HA tag in 293 cells transfected with C-terminal HA tagged gene by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:2,000) at 4°C. An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG.
HA tag was immunoprecipitated from 0.2mg of 293 whole cells lysate transfected with N-terminal HA tagged gene with ARB574 at 1:10 dilution. 2nd Ab: GAR HRP for IP 1:500 Lane 1: ARB574 IP in 293 whole cell lysate transfected with N-terminal HA tagged gene Lane 2: PBS instead of ARB574 in 293 whole cell lysate transfected with N-terminal HA tagged gene Lane 3: 293 whole cell lysate transfected with N-terminal HA tagged gene, 10 μg (input) Exposure: 60s
HA tag was immunoprecipitated from 0.2mg of 293 whole cells lysate transfected with C-terminal HA tagged gene with ARB574 at 1:10 dilution. 2nd Ab: GAR HRP for IP 1:500 Lane 1: ARB574 IP in 293 whole cell lysate transfected with C-terminal HA tagged gene Lane 2: PBS instead of ARB574 in 293 whole cell lysate transfected with C-terminal HA tagged gene Lane 3: 293 whole cell lysate transfected with C-terminal HA tagged gene, 10 μg (input) Exposure: 60s
Storage
Store at -20°C. Stable for one year from the date of shipment.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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