Histone H3 Mutation Antibody Panel (Mutated K4M, K9M, K27M, K36M), Clone RM363, RM191, RM192, RM193; 31-1326-MP
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Product Details
| Product Name | Histone H3 Mutation Antibody Panel (Mutated K4M, K9M, K27M, K36M), Clone RM363, RM191, RM192, RM193 |
| Product Description | RevMAb's Antibody Panel of rabbit monoclonal recombinant antibodies to Histone H3 K4M, Histone H3 K9M, Histone H3 K27M, Histone H3 K36M |
| Catalog No. | 31-1326-MP |
| Clone Name | RM363, RM191, RM192, RM193 |
| Product Data Sheet | Histone H3 Antibody Panel Data Sheet |
| Specificity | Histone H3 Antibody Panel reacts to Histone H3 K4M, K9M, K27M, and K36M mutants. No cross reactivity with wild type Histone H3. |
| Immunogen | A peptide corresponding to Histone H3 K4M, K9M, K27M, and K36M mutants individually. |
| Application | Western Blot ELISA Immunohistochemistry |
| Species Reactivity | All |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 25 µL each |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 1:200 – 1:2000; ELISA: 1:250 – 1:2500; IHC: 1:100 – 1:500. |
| . | |
| Immunostaining of brain tumor tissue sections using both EDTA and Citrate pretreatment methods.Clinically validated to be Positive for the H3.3 K27M Mutation by Sanger sequencing. Image courtesy of Sebastian Brandner MD, Division of Neuropathology and Dept. of Neurodegenerative Disease, UCL Institute of Neurology, London, UK |
 | Immunohistochemical staining of formalin fixed paraffin embedded (FFPE) Chondroblastoma tumor cell tissue sections using RM193 Anti-Histone H3 K36M mAb at a 1:400 dilution. Image courtesy of Adrienne Flanagan, Department of Histopathology, RONH, Stanmore, Middlesex, UK |
 | Western Blot analysis of cell lysates prepared from 293T transfected with a DNA construct encoding K9M mutant or wild type proteins of Histone H3, using anti-Histone H3 K9M rabbit monoclonal antibody clone RM191. |
 | Immunohistochemical staining of formalin fixed and paraffin embedded 293T cells transfected with a DNA construct encoding K4M mutant proteins of Histone H3, stained with anti-Histone H3 K4M rabbit monoclonal antibody clone RM363. |
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