HIV-1 p24 ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
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Brand:
CAT.NO. : AEH0078
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Size:
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Product Details
Background
The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0190 | 0.0200 | 0.0195 | |
| 3.13 | 0.0300 | 0.0310 | 0.0305 | 0.0110 |
| 6.25 | 0.0520 | 0.0500 | 0.0510 | 0.0315 |
| 12.50 | 0.1150 | 0.1140 | 0.1145 | 0.0950 |
| 25.00 | 0.2920 | 0.2800 | 0.2860 | 0.2665 |
| 50.00 | 0.7590 | 0.7000 | 0.7295 | 0.7100 |
| 100.00 | 1.8220 | 1.7240 | 1.7730 | 1.7535 |
| 200.00 | 3.2790 | 3.0910 | 3.1850 | 3.1655 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 4.6 | 18.4 | 58.9 | 4.2 | 19.5 | 59.7 |
| Standard Deviation | 0.12 | 0.59 | 4.1 | 0.21 | 0.72 | 3.8 |
| Coefficient of Variation(%) | 2.6 | 3.2 | 6.9 | 5.0 | 3.7 | 6.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of HIV-1 p24 into serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 94% to 105% with an overall mean recovery of 98%.
The recovery ranged from 94% to 105% with an overall mean recovery of 98%.
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