HLA Class II DRB1 Rabbit Monoclonal Antibody(ARA843)
Key features and details
- Reactivity:Human
- Application:WB, IHC-P
- Host:Rabbit
- Clonality:Monoclonal
- lsotype:IgG
- Target:HLA Class II DRB1
-
Brand:
CAT.NO. : ARA6665
RMB Please choose
RMB Please choose
*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。
Product Details
Background
A beta chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the alpha chain HLA-DRA, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DRB1-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells. Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes. In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins. Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance. The selection of the immunodominant epitopes follows two processing modes: 'bind first, cut/trim later' for pathogen-derived antigenic peptides and 'cut first, bind later' for autoantigens/self-peptides. The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules.
Application
|
Application |
Dilution Ratio |
|
WB |
1:1,000 |
|
IHC-P |
1:500-1:1,000 |
|
IF-Tissue |
1:100 |
Overview
|
Immunogen |
Synthetic peptide within Human HLA Class II DRB1 aa 151 - 200 / 266 |
|
Species Reactivity |
Human |
|
Molecular Weight |
Predicted band size: 30 kDa |
|
Positive Control |
Ramos cell lysate, Raji cell lysate, Daudi cell lysate, HDLM - 2 cell lysate, A375 cell lysate, human tonsil tissue, human skin tissue, human diffuse large B - cell lymphoma tissue |
|
Conjugation |
unconjugated |
|
RRID |
AB_3096784 |
|
Form |
Liquid |
|
Storage Buffer |
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide |
|
Isotype |
IgG |
|
Purification Method |
Protein A affinity purified |
Data
Western blot analysis of HLA Class II DRB1 on different lysates with Rabbit anti-HLA Class II DRB1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-HLA Class II DRB1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-HLA Class II DRB1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue with Rabbit anti-HLA Class II DRB1 antibody at 1/1,000 dilution.
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
New Products
