Human ADA ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81928
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81928-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81928-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81928-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81928-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81928-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81928-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81928-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81928-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81928-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81928-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81928-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81928 Human ADA ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ADA in serum, pleural fluid and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | ADA |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, pleural fluid and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.0%; Inter-Assay CV: 5.7% |
| 別名 | Adenosine deaminase; EC 3.5.4.4; Adenosine aminohydrolase |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ADA |
| 全名 | adenosine deaminase |
| 背景介绍 | This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq, Jul 2008] |
| 生物功能 | Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. [UniProt] |
| 产品亮点 | Related products: ADA antibodies; ADA ELISA Kits; ADA Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG81928 Human ADA ELISA Kit standard curve image
ARG81928 Human ADA ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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