Human ADAMTS13 ELISA Kit
Key features and details
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- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81483
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81483-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81483-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG81483-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81483-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81483-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81483-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81483-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81483-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81483-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81483-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81483-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81483 Human ADAMTS13 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ADAMTS13 in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | ADAMTS13 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 0.39 ng/ml |
| 样品类型 | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| 标准范围 | 0.78 - 50 ng/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.7% Inter-Assay CV: 6.1% |
| 別名 | von Willebrand factor-cleaving protease; vWF-CP; EC 3.4.24.87; ADAM-TS 13; VWFCP; A disintegrin and metalloproteinase with thrombospondin motifs 13; ADAM-TS13; vWF-cleaving protease; ADAMTS-13; C9orf8 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q76LX8 Human A disintegrin and metalloproteinase with thrombospondin motifs 13 |
|---|---|
| 基因名称 | ADAMTS13 |
| 全名 | ADAM metallopeptidase with thrombospondin type 1 motif, 13 |
| 背景介绍 | This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| 生物功能 | Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation. [UniProt] |
| 产品亮点 | Related products: ADAMTS antibodies; ADAMTS ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Glycosylated. O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS13. May also be C-glycosylated on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and also N-glycosylated. These other glycosylations can also facilitate secretion. The precursor is processed by a furin endopeptidase which cleaves off the pro-domain. [UniProt] |
检测图片 (1)
ARG81483 Human ADAMTS13 ELISA Kit standard curve image
ARG81483 Human ADAMTS13 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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