Human Adiponectin ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 0.15 pg/ml (50 μl)；3.56 pg/ml (10 μl)
Standard Curve Range: 6.86~5000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0099

RMB Please choose

Size:

96T

Trail, Bulk size or Custom requests Please contact us

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

Background

Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300 kDa high molecular weight oligomers

Typical Data

pg/ml	O.D.		Average	Corrected
0.00	0.0097	0.0102	0.0100
6.86	0.0277	0.0283	0.0280	0.0181
20.58	0.0665	0.0592	0.0629	0.0529
61.73	0.1897	0.1883	0.1890	0.1791
185.19	0.5615	0.5451	0.5533	0.5434
555.56	1.4160	1.4190	1.4175	1.4076
1666.67	2.8940	2.7930	2.8435	2.8336
5000.00	4.1026	4.1065	4.1046	4.0946

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（pg/ml）	103.3	485.3	1632.6	111.5	520.8	1724.6
Standard deviation	2.8	27.8	81.6	5.8	31.7	128.9
Coefficient of variation（%）	2.7	5.7	5.0	5.2	6.1	7.5

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human Adiponectin into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 81% to 126% with an overall mean recovery of 93%.

Sample Values

Sample Matrix

Sample Evaluated

Range (ng/ml)

Detectable

Mean of Detectable (ng/ml)

Serum

2621.9-7072.8

100

4559.1

Serum/Plasma - Thirty samples from apparently healthy volunteers were evaluated for the presence of human Adiponectin in this assay. No medical histories were available for the donors.

New Products

IDH1 Rabbit Monoclonal Antibody(ARA946)

S100 Rabbit Monoclonal Antibody(ARA945)

p63 Rabbit Monoclonal Antibody(ARA944)

Cytokeratin 5&6 Rabbit Monoclonal Antibody(ARA943)

TTF1 Rabbit Monoclonal Antibody(ARA942)

BCL-2 Rabbit Monoclonal Antibody(ARA941)