Human AGRP / Agouti-related protein ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81577
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81577-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81577-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81577-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81577-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81577-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81577-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81577-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81577-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81577-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81577-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81577-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81577 Human AGRP / Agouti-related protein ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human AGRP / Agouti-related protein in serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | AGRP / Agouti related protein |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 3.9 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants. |
| 标准范围 | 7.8 - 500 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.2% Inter-Assay CV: 5.2% |
| 別名 | Agouti-related protein; AGRT; ART; ASIP2 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | AGRP |
| 全名 | agouti related neuropeptide |
| 背景介绍 | This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity. [provided by RefSeq, Dec 2009] |
| 生物功能 | Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R (By similarity). Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner. [UniProt] |
| 产品亮点 | Related products: AGRP antibodies; AGRP ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG81577 Human AGRP / Agouti-related protein ELISA Kit standard curve image
ARG81577 Human AGRP / Agouti-related protein ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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