Human alpha 1 microglobulin ELISA Kit
Key features and details
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- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81612
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81612-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81612-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81612-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81612-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81612-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81612-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81612-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81612-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81612-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81612-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81612-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81612 Human alpha 1 microglobulin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human alpha 1 microglobulin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | alpha 1 microglobulin |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 7.8 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.3% Inter-Assay CV: 6.7% |
| 別名 | Bikunin; HCP; ITIL; ITILC; ITI; ITI-LC; UTI; Protein HC; Complex-forming glycoprotein heterogeneous in charge; EDC1; HI30; HI-30; Alpha-1 microglycoprotein; A1M; IATIL; Uronic-acid-rich protein; Protein AMBP |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | AMBP |
| 全名 | alpha-1-microglobulin/bikunin precursor |
| 背景介绍 | This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq, Jul 2008] |
| 生物功能 | Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization. Trypstatin is a trypsin inhibitor. [UniProt] |
| 产品亮点 | Related products: alpha 1 microglobulin antibodies; alpha 1 microglobulin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | The precursor is proteolytically processed into separately functioning proteins. 3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'. Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate. N- and O-glycosylated. N-glycan heterogeneity at Asn-115: Hex5HexNAc4 (major), Hex6HexNAc5 (minor) and dHex1Hex6HexNAc5 (minor). N-glycan at Asn-250: Hex5HexNAc4. O-linkage of the glycosaminoglycan, chondroitin sulfate, at Ser-215 allows cross-linking between the three polypeptide chains. [UniProt] |
检测图片 (1)
ARG81612 Human alpha 1 microglobulin ELISA Kit standard curve image
ARG81612 Human alpha 1 microglobulin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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