Human alpha Fetoprotein ELISA Kit
Key features and details
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- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG82975
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Product Details
概述
| 产品描述 | ARG82975 Human alpha Fetoprotein ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human alpha Fetoprotein in serum, plasma, cell culture supernatant samples. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | Cross-Reactivity: Not reacts with Human IL2, IL4, IL6, IL8, IL10, TNF alpha proteins; Mouse IL2, IL4, IL6, IL10, TNF beta proteins and Rat IL2, IL4, IL6, IL8, TNF beta proteins. |
| 靶点名称 | alpha Fetoprotein |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 0.78 ng/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 1.56 - 100 ng/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| 別名 | Alpha-fetoprotein; HPAFP; FETA; Alpha-fetoglobulin; AFPD; Alpha-1-fetoprotein |
应用说明
| 检测时间 | ~ 4 hours |
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属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | AFP |
| 全名 | alpha-fetoprotein |
| 背景介绍 | This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Oct 2019] |
| 生物功能 | Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 翻译后修饰 | Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. Sulfated. [UniProt] |
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