Human Alpha-Synuclein ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0191
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Size:
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Product Details
Background
alpha -Synuclein (SNCA) is a cytoplasmic protein that is predominantly expressed in the central nervous system. It is a minor protein found in amyloid deposition that is a hallmark of Alzheimer's disease. Defects in SNCA are associated with familial Parkinson's disease.
Typical data
|
ng/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0108 |
0.0105 |
0.0107 |
|
|
0.78 |
0.0171 |
0.0166 |
0.0169 |
0.0062 |
|
1.56 |
0.0328 |
0.0294 |
0.0311 |
0.0205 |
|
3.13 |
0.0793 |
0.0817 |
0.0805 |
0.0699 |
|
6.25 |
0.2713 |
0.2822 |
0.2768 |
0.2661 |
|
12.50 |
1.0200 |
1.0220 |
1.0210 |
1.0104 |
|
25.00 |
3.0020 |
3.0060 |
3.0040 |
2.9934 |
|
50.00 |
4.5515 |
4.5446 |
4.5481 |
4.5374 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(ng/ml) |
3.1 |
8.6 |
22.3 |
2.7 |
7.8 |
20.3 |
|
Standard Deviation |
0.1 |
0.2 |
0.9 |
0.1 |
0.2 |
0.5 |
|
Coefficient of Variation(%) |
3.8 |
2.9 |
4.0 |
2.9 |
2.8 |
2.6 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Alpha-Synuclein into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 120% with an overall mean recovery of 99%.
The recovery ranged from 83% to 120% with an overall mean recovery of 99%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 126.53-645.86 | 100 | 269.28 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Alpha-Synuclein in this assay. No medical histories were available for the donors.
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