Human Angiogenin ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEHY0190
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Size:
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Product Details
Background
Angiogenin (ANG) is a secreted protein that belongs to the pancreatic ribonuclease family. It was initially purified based on its ability to initiate vascularization in the chicken embryo chorioallantoic membrane from serum-free media conditioned by growth of a human adenocarcinoma cell line HT-29. A number of other tumors, as well as normal cell lines, also secrete Angiogenin. Angiogenin is present in normal human plasma.
Typical data
|
ng/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0123 |
0.0123 |
0.0123 |
|
|
0.41 |
0.0423 |
0.0436 |
0.0430 |
0.0307 |
|
1.02 |
0.0915 |
0.0921 |
0.0918 |
0.0795 |
|
2.56 |
0.2093 |
0.2128 |
0.2111 |
0.1988 |
|
6.40 |
0.5093 |
0.5195 |
0.5144 |
0.5021 |
|
16.00 |
1.2320 |
1.2210 |
1.2265 |
1.2142 |
|
40.00 |
2.5030 |
2.4640 |
2.4835 |
2.4712 |
|
100.00 |
3.4960 |
3.4790 |
3.4875 |
3.4752 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(ng/ml) |
2.2 |
9.9 |
34.2 |
2.4 |
10.6 |
35.4 |
|
Standard Deviation |
0.1 |
0.1 |
0.6 |
0.0 |
0.2 |
0.6 |
|
Coefficient of Variation(%) |
2.3 |
1.4 |
1.6 |
1.1 |
1.7 |
1.7 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Angiogenin into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 80% to 119% with an overall mean recovery of 96%
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 115.46-277.38 | 100 | 168.44 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Angiogenin in this assay. No medical histories were available for the donors.
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