Human Angiopoietin like 4 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81489
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81489-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81489-002 | Standard | 2 X 40 ng/vial | 4°C |
| ARG81489-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81489-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81489-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81489-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81489-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81489-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81489-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81489-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81489-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81489 Human Angiopoietin like 4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Angiopoietin like 4 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | Angiopoietin like 4 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 312.5 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 625 - 40000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.6% Inter-Assay CV: 6.1% |
| 別名 | UNQ171; NL2; pp1158; FIAF; Angiopoietin-like protein 4; TGQTL; Hepatic fibrinogen/angiopoietin-related protein; Angiopoietin-related protein 4; ARP4; PGAR; HFARP; HARP |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ANGPTL4 |
| 全名 | angiopoietin-like 4 |
| 背景介绍 | This gene encodes a glycosylated, secreted protein containing a C-terminal fibrinogen domain. The encoded protein is induced by peroxisome proliferation activators and functions as a serum hormone that regulates glucose homeostasis, lipid metabolism, and insulin sensitivity. This protein can also act as an apoptosis survival factor for vascular endothelial cells and can prevent metastasis by inhibiting vascular growth and tumor cell invasion. The C-terminal domain may be proteolytically-cleaved from the full-length secreted protein. Decreased expression of this gene has been associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4. [provided by RefSeq, Sep 2013] |
| 生物功能 | Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation (By similarity). [UniProt] |
| 产品亮点 | Related products: Angiopoietin antibodies; Angiopoietin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | N-glycosylated. [UniProt] |
检测图片 (1)
ARG81489 Human Angiopoietin like 4 ELISA Kit standard curve image
ARG81489 Human Angiopoietin like 4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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