Human ANP / Atrial Natriuretic Peptide ELISA Kit
Key features and details
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- 应用:
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- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
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Brand:
CAT.NO. : ARG81988
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81988-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81988-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81988-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81988-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81988-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81988-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81988-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81988-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81988-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81988-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81988-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81988 Human ANP / Atrial Natriuretic Peptide ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ANP / Atrial Natriuretic Peptide in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | ANP / Atrial Natriuretic Peptide |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.8%; Inter-Assay CV: 7.4% |
| 別名 | CDD; Natriuretic peptides A; ANF; Cardiodilatin; Cardiodilatin-related peptide; Atrial natriuretic peptide; Prepronatriodilatin; ATFB6; CDP; ATRST2; ANP; CDD-ANF; PND |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NPPA |
| 全名 | natriuretic peptide A |
| 背景介绍 | The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq, Sep 2009] |
| 生物功能 | Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. [UniProt] |
| 产品亮点 | Related products: Atrial Natriuretic Peptide antibodies; Atrial Natriuretic Peptide ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Cleaved by CORIN upon secretion to produce the functional hormone. Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity. [UniProt] |
检测图片 (1)
ARG81988 Human ANP / Atrial Natriuretic Peptide ELISA Kit standard curve image
ARG81988 Human ANP / Atrial Natriuretic Peptide ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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