Human APLP1 ELISA Kit
Key features and details
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- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
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Brand:
CAT.NO. : ARG82747
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82747-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82747-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82747-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82747-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82747-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82747-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82747-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82747-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82747-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82747-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82747-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82747 Human APLP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human APLP1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | APLP1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.3% Inter-Assay CV: 6.6% |
| 別名 | APLP; Amyloid-like protein 1; APLP-1 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | APLP1 |
| 全名 | amyloid beta (A4) precursor-like protein 1 |
| 背景介绍 | This gene encodes a member of the highly conserved amyloid precursor protein gene family. The encoded protein is a membrane-associated glycoprotein that is cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. This cleavage liberates an intracellular cytoplasmic fragment that may act as a transcriptional activator. The encoded protein may also play a role in synaptic maturation during cortical development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| 生物功能 | May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I. The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. C30: Cytoplasm. Note=C-terminally processed in the Golgi complex. [UniProt] |
| 产品亮点 | Related products: APLP1 antibodies; APLP1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Proteolytically cleaved by caspases during neuronal apoptosis. Cleaved, in vitro, at Asp-620 by caspase-3 (By similarity). N- and O-glycosylated. O-glycosylation with core 1 or possibly core 8 glycans. Glycosylation on Ser-227 is the preferred site to Thr-228. [UniProt] |
检测图片 (1)
ARG82747 Human APLP1 ELISA Kit standard curve image
ARG82747 Human APLP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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