Human B7-H2/CD257 ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
Product Details
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0144 |
0.0126 |
0.0135 |
|
|
13.72 |
0.0336 |
0.0343 |
0.0340 |
0.0205 |
|
41.15 |
0.0807 |
0.0725 |
0.0766 |
0.0631 |
|
123.46 |
0.1894 |
0.2274 |
0.2084 |
0.1949 |
|
370.37 |
0.4951 |
0.5551 |
0.5251 |
0.5116 |
|
1111.11 |
1.2880 |
1.3600 |
1.3240 |
1.3105 |
|
3333.33 |
2.7260 |
2.8360 |
2.7810 |
2.7675 |
|
10000.00 |
4.1722 |
4.2258 |
4.1990 |
4.1855 |
|
Intra-assay Precision |
|
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
|
22 |
22 |
22 |
6 |
6 |
6 |
||
|
Average（pg/ml） |
246.6 |
1218.8 |
4221.3 |
132.7 |
829.4 |
3607.5 |
|
|
Standard deviation |
19.3 |
97.6 |
239.4 |
5.4 |
63.9 |
268.3 |
|
|
Coefficient of variation（%） |
7.8 |
8.0 |
5.7 |
4.0 |
7.7 |
7.4 |
|
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision
The spike recovery was evaluated by spiking 3 levels of human B7-H2/CD275 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 72% to 107% with an overall mean recovery of 87%.
|
Sample Matrix |
Sample Evaluated |
Range (ng/ml) |
Detectable % |
Mean of Detectable (ng/ml) |
|
Serum |
30 |
0.07-193.97 |
100 |
106.56 |
Serum/Plasma - Thirty samples from apparently healthy volunteers were evaluated for the presence of B7-H2/CD275 in this assay. No medical histories were available for the donors.
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