Human CCL25 ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0117
RMB Please choose
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Size:
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Product Details
Background
CCL25 (thymus-expressed chemokine) is a novel CC chemokine that is distantly related (approximately 20% amino acid sequence identity) to other CC chemokines. Human CCL25 cDNA encodes a 150 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 127 residue mature protein. Mouse CCL25 cDNA has also been cloned and shown to encode a 144 amino acid protein that exhibits 49% amino acid sequence identity to human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Recombinant human and mouse CCL25 have been shown to be chemotactic for activated macrophages, dendritic cells and thymocytes. CCL25 has also been shown to be a specific agonist for the orphan receptor called GPR-9-6, now named CCR9.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0067 | 0.0063 | 0.0065 | |
| 1.37 | 0.0212 | 0.0206 | 0.0209 | 0.0144 |
| 4.12 | 0.0535 | 0.0512 | 0.0524 | 0.0459 |
| 12.35 | 0.1545 | 0.1510 | 0.1528 | 0.1463 |
| 37.04 | 0.4450 | 0.4292 | 0.4371 | 0.4306 |
| 111.11 | 1.2110 | 1.2380 | 1.2245 | 1.2180 |
| 333.33 | 2.6520 | 2.6360 | 2.6440 | 2.6375 |
| 1000.00 | 4.0170 | 4.0352 | 4.0261 | 4.0196 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 23.6 | 103.0 | 292.7 | 24.1 | 115.1 | 349.9 |
| Standard Deviation | 1.0 | 5.4 | 18.0 | 1.3 | 1.8 | 19.4 |
| Coefficient of Variation(%) | 4.4 | 5.3 | 6.2 | 5.3 | 1.6 | 5.5 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CCL25 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 77% to 98% with an overall mean recovery of 84%.
The recovery ranged from 77% to 98% with an overall mean recovery of 84%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | 0.12-5.85 | 100 | 3.15 |
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