Human BCMA/TNFRSF17 ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 0.45 pg/ml (50 μl)；1.52 pg/ml (10 μl)
Standard Curve Range: 6.86~5000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0143

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Size:

96T

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Product Details

Background

BCMA, B cell maturation antigen, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0088	0.0086	0.0087
6.86	0.0244	0.0247	0.0246	0.0158
20.58	0.0611	0.0643	0.0627	0.0540
61.73	0.2083	0.2159	0.2121	0.2034
185.19	0.6262	0.6201	0.6232	0.6144
555.56	1.8500	1.9220	1.8860	1.8773
1666.67	3.2090	3.2870	3.2480	3.2393
5000.00	3.6870	3.6950	3.6910	3.6823

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（pg/ml）	97.3	531.3	963.5	102.3	532.2	1496.3
Standard deviation	3.7	26.4	17.4	1.9	16.1	28.5
Coefficient of variation（%）	3.8	5.0	1.8	1.9	3.0	1.9

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human BCMA/TNFRSF17 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 84% to 106% with an overall mean recovery of 93%.

Sample Values

Sample Matrix

Sample

Evaluated

Range

(pg/ml)

Detectable

Mean of Detectable

(pg/ml)

Serum

1715.39-3905.61

100.0

2432.79

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of BCMA/TNFRSF17 in this assay. No medical histories were available for the donors.

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